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Image Search Results
Journal: Oncotarget
Article Title: A novel curcumin analog inhibits canonical and non-canonical functions of telomerase through STAT3 and NF-κB inactivation in colorectal cancer cells
doi: 10.18632/oncotarget.27000
Figure Lengend Snippet: Transcription factor STAT3 binding to hTERT promoter region was tested with chromatin immunoprecipitation assay. ( A ) Both HCT116 and DLD1 were treated with compound 19 and applied to ChIP assay. Densiometric values of DNA bands were measured with Image J soft ware with the untreated control cell set as 1.0. ( B ) EMSA assay was performed with compound 19 treated HCT116 and DLD1. ( C ) Telomerase activities were measured by TRAP PCR reaction conjugated to Elisa assay. ( D ) HCT116 and DLD1 cells were treated with compound 19 and applied for telomere length quantification qPCR assays.
Article Snippet: To measure the telomeric DNA length changes, we used
Techniques: Binding Assay, Chromatin Immunoprecipitation, Control, Enzyme-linked Immunosorbent Assay